Bu1 Antibody (AV20) [Biotin]

Novus Biologicals | Catalog # NBP1-28343

Novus Biologicals

Key Product Details

Species Reactivity

Chicken

Applications

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AV20
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Product Specifications

Immunogen

Chicken peripheral blood lymphocytes and splenocytes

Reactivity Notes

Chicken

Specificity

Chicken Bu-1 (chB6), Mr 70-75 kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Bu1 Antibody (AV20) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bu1

Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cellassociated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. (1-2) MAb AV20 does not cross react with turkey cells when analyzed by immunofluorescent staining and flow cytometry. (3)

Alternate Names

B6.1, B6.2, Bu 1a, Bu 1b, LOC396098

Additional Bu1 Products

Product Documents for Bu1 Antibody (AV20) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bu1 Antibody (AV20) [Biotin]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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