c-Abl Antibody (8E9)

Novus Biologicals | Catalog # NBP2-49695

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E9
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Product Specifications

Immunogen

Recombinant Abl protein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse c-Abl Antibody (8E9) (NBP2-49695) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for c-Abl Antibody (8E9)

Western Blot: c-Abl Antibody (8E9) [NBP2-49695]

Western Blot: c-Abl Antibody (8E9) [NBP2-49695]

Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.
Immunocytochemistry/ Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695]

Immunocytochemistry/ Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695]

Immunocytochemistry/Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695] - taining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695]

Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695]

Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.

Applications for c-Abl Antibody (8E9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Immunoprecipitation

2 ug/mg

Western Blot

1 ug/mL

Reviewed Applications

Read 1 review rated 4 using NBP2-49695 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: c-Abl

c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.

Long Name

Abelson Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

ABL1, BCR-ABL1, bcr/abl, cAbl, JTK7

Gene Symbol

ABL1

Additional c-Abl Products

Product Documents for c-Abl Antibody (8E9)

Certificate of Analysis

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Product Specific Notices for c-Abl Antibody (8E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for c-Abl Antibody (8E9) (1)

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  • c-Abl Antibody (8E9)
    Name: Pauline Jeannot
    Application: Immunocytochemistry
    Sample Tested: Human primary astrocytes
    Species: Human
    Verified Customer | Posted 11/16/2023
    Human primary astrocytes stained with c-Abl antibody (1:100 dilution, 4% PFA fixation)
    c-Abl Antibody (8E9) NBP2-49695

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Protocols

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