Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, Immunoassay, Sandwich ELISA

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CC34

Format

BSA Free
View all C-Peptide Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Reacts with rat c-peptide I, rat c-peptide II, mouse c-peptide II and mouse c-peptide I. Does not react with rat proinsulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for C-Peptide Antibody (CC34) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Sandwich ELISA

1:100 - 1:2000
Application Notes
Can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples. The C-Peptide Antibody (CC34) NBP1-05433 can be use as the capture antibody with the use of detection antibody C-Peptide Antibody (CII-11) NBP1-05432 in Sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: C-Peptide

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Long Name

Proinsulin C-Peptide

Alternate Names

C-P, Insulin C-Peptide, Proinsulin C-Peptide

Entrez Gene IDs

16334 (Mouse); 24506 (Rat)

Gene Symbol

INS

Additional C-Peptide Products

Product Documents for C-Peptide Antibody (CC34) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for C-Peptide Antibody (CC34) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for C-Peptide Antibody (CC34) - BSA Free

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Protocols

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