Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all c-Rel Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 569 and 619 of human reticuloendotheliosis viral oncogene homolog using the numbering given in entry NP_002899.1 (GeneID 5966).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for c-Rel Antibody - BSA Free

Western Blot: c-Rel Antibody [NB100-97834]

Western Blot: c-Rel Antibody [NB100-97834]

Western Blot: c-Rel Antibody [NB100-97834] - Detection of Human c-Rel by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-c-Rel antibody NB100-97834 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. c-Rel was also immunoprecipitated by rabbit anti-c-Rel antibody NB100-97833, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Applications for c-Rel Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: c-Rel

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM]

Long Name

Cellular Repressor of E1A-stimulated Genes

Alternate Names

cRel, REL

Entrez Gene IDs

5966 (Human)

Gene Symbol

REL

UniProt

NP_002899.1 (Human)

Additional c-Rel Products

Product Documents for c-Rel Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for c-Rel Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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