C10orf82 Antibody (OTI1G4)
Novus Biologicals | Catalog # NBP2-02828
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1G4
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Product Specifications
Immunogen
Full length human recombinant protein of human C10orf82 (NP_653262) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for C10orf82 Antibody (OTI1G4)
![Western Blot: C10orf82 Antibody (OTI1G4) [NBP2-02828]](https://resources.rndsystems.com/images/products/C10orf82-Antibody-OTI1G4-Western-Blot-NBP2-02828-img0001.jpg "Western Blot: C10orf82 Antibody (OTI1G4) [NBP2-02828]")
Western Blot: C10orf82 Antibody (OTI1G4) [NBP2-02828]
Western Blot: C10orf82 Antibody (OTI1G4) [NBP2-02828] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C10orf82 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C10orf82.![Immunocytochemistry/ Immunofluorescence: C10orf82 Antibody (OTI1G4) [NBP2-02828]](https://resources.rndsystems.com/images/products/C10orf82-Antibody-OTI1G4-Immunocytochemistry-Immunofluorescence-NBP2-02828-img0002.jpg "Immunocytochemistry/ Immunofluorescence: C10orf82 Antibody (OTI1G4) [NBP2-02828]")
Immunocytochemistry/ Immunofluorescence: C10orf82 Antibody (OTI1G4) [NBP2-02828]
Immunocytochemistry/Immunofluorescence: C10orf82 Antibody (OTI1G4) [NBP2-02828] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C10orf82.Applications for C10orf82 Antibody (OTI1G4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.43 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: C10orf82
Alternate Names
chromosome 10 open reading frame 82, Em:AC016825.4, FLJ40268, hypothetical protein LOC143379, MGC33547
Entrez Gene IDs
143379 (Human)
Gene Symbol
C10ORF82
Additional C10orf82 Products
Product Documents for C10orf82 Antibody (OTI1G4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for C10orf82 Antibody (OTI1G4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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