C1GALT1 Antibody (1F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00056913-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F1

Format

Azide and BSA Free
View all C1GalT1 Primary Antibodies »

Product Specifications

Immunogen

C1GALT1 (NP_064541, 264 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETFHPFVPEHHLIKGYLPRTFWYWNYNYYPPVEGPGCCSDLAVSFHYVDSTTMYELEYLVYHLRPYGYLYRYQPTLPERILKEISQANKNEDTKVKLGNP

Specificity

C1GALT1 - core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for C1GALT1 Antibody (1F1) - Azide and BSA Free

Western Blot: C1GALT1 Antibody (1F1) [H00056913-M01]

Western Blot: C1GALT1 Antibody (1F1) [H00056913-M01]

Western Blot: C1GALT1 Antibody (1F1) [H00056913-M01] - C1GALT1 monoclonal antibody (M01), clone 1F1 Analysis of C1GALT1 expression in HeLa.
Immunohistochemistry-Paraffin: C1GALT1 Antibody (1F1) [H00056913-M01]

Immunohistochemistry-Paraffin: C1GALT1 Antibody (1F1) [H00056913-M01]

Immunohistochemistry-Paraffin: C1GALT1 Antibody (1F1) [H00056913-M01] - Analysis of monoclonal antibody to C1GALT1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.
ELISA: C1GALT1 Antibody (1F1) [H00056913-M01]

ELISA: C1GALT1 Antibody (1F1) [H00056913-M01]

ELISA: C1GALT1 Antibody (1F1) [H00056913-M01] - Detection limit for recombinant GST tagged C1GALT1 is approximately 0.3ng/ml as a capture antibody.

Applications for C1GALT1 Antibody (1F1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: C1GalT1

The common core 1 O-glycan structure Gal-beta-1-3GalNAc-R is a precursor for many extended mucin-type O-glycan structures in animal cell surface and secreted glycoproteins. Core 1 is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R by core 1 beta-3-galactosyltransferase (C1GALT1) (Ju et al., 2002 [PubMed 11677243]).[supplied by OMIM]

Long Name

Core 1 Synthase, Glycoprotein-N-Acetylgalactosamine 3-Beta-Galactosyltransferase, 1

Alternate Names

B3Gal-T8, Core 1 Beta3-Gal-T1, Core 1 O-Glycan T-Synthase

Entrez Gene IDs

56913 (Human)

Gene Symbol

C1GALT1

UniProt

NP_064541 (Human)

Additional C1GalT1 Products

Product Documents for C1GALT1 Antibody (1F1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for C1GALT1 Antibody (1F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for C1GALT1 Antibody (1F1) - Azide and BSA Free

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Protocols

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FAQs

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