CA19-9/Sialyl Lewis A Antibody (SPM588)

Novus Biologicals | Catalog # NBP2-45199

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # SPM588
View all CA19-9 Primary Antibodies »

Product Specifications

Immunogen

Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).

Localization

Cytoplasmic

Marker

GI Tumor Marker

Specificity

CA19-9, a carbohydrate epitope expressed on a high MW (400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This monoclonal antibody is superb for staining of formalin-fixed, paraffin-embedded tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11589)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CA19-9/Sialyl Lewis A Antibody (SPM588)

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199] - Human thyroid carcinoma tissue section. Fixed with 4% formaldehyde, incubated O/N at 4C with primary antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199] - Human Colon Carcinoma stained with CA19-9 Monoclonal Antibody (SPM588)).

Applications for CA19-9/Sialyl Lewis A Antibody (SPM588)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1 - 2 ug/mL for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-45199 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CA19-9

Mucin glucoprotein is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9

Additional CA19-9 Products

Product Documents for CA19-9/Sialyl Lewis A Antibody (SPM588)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CA19-9/Sialyl Lewis A Antibody (SPM588)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for CA19-9/Sialyl Lewis A Antibody (SPM588) (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CA19-9/Sialyl Lewis A Antibody (SPM588)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CA19-9/Sialyl Lewis A Antibody (SPM588)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Thyroid carcinoma
    Species: Human
    Verified Customer | Posted 10/14/2021
    Thyroid carcinoma
    4% formaldehyde fixed paraffin sections, incubated O/N in 4 C with primary antibody.
    CA19-9/Sialyl Lewis A Antibody (SPM588) NBP2-45199

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies