Cadherin-11 Antibody (3C8) - Azide and BSA Free

Novus Biologicals | Catalog # H00001009-M05-100ug

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C8

Format

Azide and BSA Free
View all Cadherin-11 Primary Antibodies »

Product Specifications

Immunogen

CDH11 (P55287, 54 a.a. ~ 612 a.a) partial recombinant protein with Fc tag at C terminal. MW of the Fc tag alone is 50 KDa. GWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVMITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKIAVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVPVAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQPIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTVRDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILN

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cadherin-11 Antibody (3C8) - Azide and BSA Free

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection against CHD11-His tag (61.49 KDa).
Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml]
Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in MCF-7.
Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in human kidney.
Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.
ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug]

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.

Applications for Cadherin-11 Antibody (3C8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cadherin-11

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Entrez Gene IDs

1009 (Human)

Gene Symbol

CDH11

OMIM

600023 (Human)

UniProt

P55287 (Human)

Additional Cadherin-11 Products

Product Documents for Cadherin-11 Antibody (3C8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cadherin-11 Antibody (3C8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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Protocols

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FAQs

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