Cadherin-16 Antibody (CDH16/2125)

Novus Biologicals | Catalog # NBP2-79722

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CDH16/2125
View all Cadherin-16 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 371-507) of human Cadherin-16 protein (exact sequence is proprietary) (Uniprot: O75309)

Localization

Cell Surface, Cytoplasm

Marker

Renal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79856)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cadherin-16 Antibody (CDH16/2125)

Western Blot: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Western Blot: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Western Blot: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722] - Western Blot Analysis of Human Kidney lysate using Cadherin-16 Antibody (CDH16/2125).
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722] - Formalin-fixed, paraffin-embedded human kidney stained with Biotin-conjugated Cadherin-16Mouse Monoclonal Antibody (Cadherin-16/2125).
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Cadherin-16 Antibody (CDH16/2125).
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Cadherin-16 Antibody (CDH16/2125).
Protein Array: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Protein Array: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722]

Protein Array: Cadherin-16 Antibody (CDH16/2125) [NBP2-79722] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cadherin-16 Antibody (CDH16/2125). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Cadherin-16 Antibody (CDH16/2125)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cadherin-16

Cadherin 16 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Alternate Names

cadherin 16, KSP-cadherin, cadherin-16, Kidney-specific cadherin, KSP-cadherin

Gene Symbol

CDH16

UniProt

O75309 (Human)

Additional Cadherin-16 Products

Product Documents for Cadherin-16 Antibody (CDH16/2125)

Certificate of Analysis

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Product Specific Notices for Cadherin-16 Antibody (CDH16/2125)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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