Calcein AM Cell Viability Assay
Calcein AM Cell Viability Assay Summary
• Suitable for proliferating and non-proliferating cells.
• Ideal for both suspension and adherent cells.
• Non-radioactive assay.
• Rapid (no solubilization step as in a conventional MTT assay).
• Ideal for high-throughput experiments.
• Better retention and brightness compared to other fluorescent compounds
Why Use the Calcein AM Cell Viability Assay?
Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
• Calcein AM
• 10X Calcein AM DW Buffer
For research use only. Not for diagnositic use.
Citations for Calcein AM Cell Viability Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
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Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro
Authors: JA Hörauf, S Kany, A Janicova, B Xu, T Vrdoljak, R Sturm, IR Dunay, L Martin, B Relja
Int J Mol Sci, 2020;21(9):. 2020
Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism
Authors: J Lee, AE Yesilkanal, JP Wynne, C Frankenber, J Liu, J Yan, M Elbaz, DC Rabe, FD Rustandy, P Tiwari, EA Grossman, PC Hart, C Kang, SM Sanderson, J Andrade, DK Nomura, MG Bonini, JW Locasale, MR Rosner
Nature, 2019;0(0):. 2019
Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells
Authors: P Nguemgo Ko, GA Rezniczek, A Kochanneck, B Priesch-Gr, T Hero, IA Adamietz, H Bühler
PLoS ONE, 2018;13(6):e0198508. 2018
Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer
Authors: MM Olcina, NG Balanis, RK Kim, BA Aksoy, J Kodysh, MJ Thompson, J Hammerbach, TG Graeber, AJ Giaccia
Cell Rep, 2018;25(13):3721-3732.e6. 2018
Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
Aging Dis, 2017;8(2):203-214. 2017
Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1? activation.
Authors: Jonathan D Diedrich, Erandi Rajagurub, Mackenzie K Herroon, Gargi Mahapatra, Maik Hüttemann, Izabela Podgorski
Oncotarget, 2016;0(0):1949-2553. 2016
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
J Leukoc Biol, 2014;96(3):365-75. 2014
Prion protein interaction with soil humic substances: environmental implications.
Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
PLoS ONE, 2014;9(6):e100016. 2014
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