Calcein AM Cell Viability Assay

Catalog # Availability Size / Price Qty
4892-010-K
Product Details
Citations (8)
FAQs
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Calcein AM Cell Viability Assay Summary

The Calcein AM Cell Viability Assay provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity.

Key Benefits

• Suitable for proliferating and non-proliferating cells.
• Ideal for both suspension and adherent cells.
• Non-radioactive assay.
• Rapid (no solubilization step as in a conventional MTT assay).
• Ideal for high-throughput experiments.
• Better retention and brightness compared to other fluorescent compounds

Why Use the Calcein AM Cell Viability Assay?

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.

  • Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.

    What is Calcein AM?

    Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

    Kit Contents

    • Calcein AM
    • 10X Calcein AM DW Buffer

    Specifications

    Shipping Conditions
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
    Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
    Species
    N/A

    Limitations

    For research use only. Not for diagnositic use.

  • Product Datasheets

    Citations for Calcein AM Cell Viability Assay

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    8 Citations: Showing 1 - 8
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    1. Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro
      Authors: JA Hörauf, S Kany, A Janicova, B Xu, T Vrdoljak, R Sturm, IR Dunay, L Martin, B Relja
      Int J Mol Sci, 2020;21(9):.  2020
    2. Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism
      Authors: J Lee, AE Yesilkanal, JP Wynne, C Frankenber, J Liu, J Yan, M Elbaz, DC Rabe, FD Rustandy, P Tiwari, EA Grossman, PC Hart, C Kang, SM Sanderson, J Andrade, DK Nomura, MG Bonini, JW Locasale, MR Rosner
      Nature, 2019;0(0):.  2019
    3. Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells
      Authors: P Nguemgo Ko, GA Rezniczek, A Kochanneck, B Priesch-Gr, T Hero, IA Adamietz, H Bühler
      PLoS ONE, 2018;13(6):e0198508.  2018
    4. Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer
      Authors: MM Olcina, NG Balanis, RK Kim, BA Aksoy, J Kodysh, MJ Thompson, J Hammerbach, TG Graeber, AJ Giaccia
      Cell Rep, 2018;25(13):3721-3732.e6.  2018
    5. Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
      Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
      Aging Dis, 2017;8(2):203-214.  2017
    6. Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1? activation.
      Authors: Jonathan D Diedrich, Erandi Rajagurub, Mackenzie K Herroon, Gargi Mahapatra, Maik Hüttemann, Izabela Podgorski
      Oncotarget, 2016;0(0):1949-2553.  2016
    7. Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
      Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
      J Leukoc Biol, 2014;96(3):365-75.  2014
    8. Prion protein interaction with soil humic substances: environmental implications.
      Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
      PLoS ONE, 2014;9(6):e100016.  2014

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