Calcein AM Cell Viability Assay

  (2 citations)     
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Calcein AM

Calcein AM provides a simple, rapid, and accurate method to measure cell viability and/or cytotoxicity. Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces Calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates (Catalog # DY991) can be quantified in less than two hours using a fluorescence plate reader with excitation at 490 nm and emission at 520 nm. Kit contains reagents for ~1000 tests.

Label: Calcein fluorescence
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: ~1000 tests


TACS is a registered trademark of Trevigen, Inc.


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Preparation and Storage
  • Stability & Storage
    The product requires storage at -20 to -70 °C and room temperature.  Consult the product insert for specific storage temperatures. Do not use past expiration date.
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R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
    Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
    Aging Dis, 2017;8(2):203-214.  2017
  2. Prion protein interaction with soil humic substances: environmental implications.
    Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
    PLoS ONE, 2014;9(6):e100016.  2014

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