Calcein AM Cell Viability Assay
Calcein AM Cell Viability Assay Summary
• Suitable for proliferating and non-proliferating cells.
• Ideal for both suspension and adherent cells.
• Non-radioactive assay.
• Rapid (no solubilization step as in a conventional MTT assay).
• Ideal for high-throughput experiments.
• Better retention and brightness compared to other fluorescent compounds
Why Use the Calcein AM Cell Viability Assay?
Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
• Calcein AM
• 10X Calcein AM DW Buffer
For research use only. Not for diagnostic use.
Citations for Calcein AM Cell Viability Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 9
Filter your results:
Phosphorylation of Microglial IRF5 and IRF4 by IRAK4 Regulates Inflammatory Responses to Ischemia
Authors: C Ngwa, AA Mamun, Y Xu, R Sharmeen, F Liu
Cells, 2021;10(2):. 2021
Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro
Authors: JA Hörauf, S Kany, A Janicova, B Xu, T Vrdoljak, R Sturm, IR Dunay, L Martin, B Relja
Int J Mol Sci, 2020;21(9):. 2020
Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism
Authors: J Lee, AE Yesilkanal, JP Wynne, C Frankenber, J Liu, J Yan, M Elbaz, DC Rabe, FD Rustandy, P Tiwari, EA Grossman, PC Hart, C Kang, SM Sanderson, J Andrade, DK Nomura, MG Bonini, JW Locasale, MR Rosner
Nature, 2019;0(0):. 2019
Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells
Authors: P Nguemgo Ko, GA Rezniczek, A Kochanneck, B Priesch-Gr, T Hero, IA Adamietz, H Bühler
PLoS ONE, 2018;13(6):e0198508. 2018
Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer
Authors: MM Olcina, NG Balanis, RK Kim, BA Aksoy, J Kodysh, MJ Thompson, J Hammerbach, TG Graeber, AJ Giaccia
Cell Rep, 2018;25(13):3721-3732.e6. 2018
Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
Aging Dis, 2017;8(2):203-214. 2017
Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1? activation.
Authors: Jonathan D Diedrich, Erandi Rajagurub, Mackenzie K Herroon, Gargi Mahapatra, Maik Hüttemann, Izabela Podgorski
Oncotarget, 2016;0(0):1949-2553. 2016
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
J Leukoc Biol, 2014;96(3):365-75. 2014
Prion protein interaction with soil humic substances: environmental implications.
Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
PLoS ONE, 2014;9(6):e100016. 2014
No product specific FAQs exist for this product, however you mayView all FAQs
Reviews for Calcein AM Cell Viability Assay
There are currently no reviews for this product. Be the first to review Calcein AM Cell Viability Assay and earn rewards!
Have you used Calcein AM Cell Viability Assay?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image