Calcein AM Cell Viability Assay
Calcein AM Cell Viability Assay Summary
Calcein AM provides a simple, rapid, and accurate method to measure cell viability and/or cytotoxicity. Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces Calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates (Catalog # DY991) can be quantified in less than two hours using a fluorescence plate reader with excitation at 490 nm and emission at 520 nm. Kit contains reagents for ~1000 tests.
Label: Calcein fluorescence
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: ~1000 tests
TACS is a registered trademark of Trevigen, Inc.
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Citations for Calcein AM Cell Viability Assay
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Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells
Authors: P Nguemgo Ko, GA Rezniczek, A Kochanneck, B Priesch-Gr, T Hero, IA Adamietz, H Bühler
PLoS ONE, 2018;13(6):e0198508. 2018
Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
Aging Dis, 2017;8(2):203-214. 2017
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
J Leukoc Biol, 2014;96(3):365-75. 2014
Prion protein interaction with soil humic substances: environmental implications.
Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
PLoS ONE, 2014;9(6):e100016. 2014
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