Calcein AM Cell Viability Assay

Catalog # Availability Size / Price Qty
4892-010-K
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Calcein AM Cell Viability Assay Summary

The Calcein AM Cell Viability Assay provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity.

Key Benefits

• Suitable for proliferating and non-proliferating cells.
• Ideal for both suspension and adherent cells.
• Non-radioactive assay.
• Rapid (no solubilization step as in a conventional MTT assay).
• Ideal for high-throughput experiments.
• Better retention and brightness compared to other fluorescent compounds

Why Use the Calcein AM Cell Viability Assay?

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.

  • Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.

    What is Calcein AM?

    Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

    Kit Contents

    • Calcein AM
    • 10X Calcein AM DW Buffer

    Specifications

    Shipping Conditions
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
    Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
    Species
    N/A

    Limitations

    For research use only. Not for diagnositic use.

  • Product Datasheets

    Reagents Provided

    FAQs

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