Calcein AM Cell Viability Assay

Discontinued Product

4892-010-K has been discontinued.
View all Cell Viability/Proliferation Assays products.
Product Details
Citations (13)
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Calcein AM Cell Viability Assay Summary

The Calcein AM Cell Viability Assay provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity.
 

Key Benefits

• Suitable for proliferating and non-proliferating cells.
• Ideal for both suspension and adherent cells.
• Non-radioactive assay.
• Rapid (no solubilization step as in a conventional MTT assay).
• Ideal for high-throughput experiments.
• Better retention and brightness compared to other fluorescent compounds

Why Use the Calcein AM Cell Viability Assay?

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.

  • Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.

    What is Calcein AM?

    Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

    Kit Contents

    • Calcein AM
    • 10X Calcein AM DW Buffer

    Specifications

    Shipping Conditions
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
    Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
    Species
    N/A

    Limitations

    For research use only. Not for diagnostic use.

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    Citations for Calcein AM Cell Viability Assay

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    13 Citations: Showing 1 - 10
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    1. Phosphorylation of Microglial IRF5 and IRF4 by IRAK4 Regulates Inflammatory Responses to Ischemia
      Authors: C Ngwa, AA Mamun, Y Xu, R Sharmeen, F Liu
      Cells, 2021;10(2):.  2021
    2. Anti-inflammatory Effects of Alcohol Are Associated with JNK-STAT3 Downregulation in an In Vitro Inflammation Model in HepG2 Cells
      Authors: K Mörs, R Sturm, JA Hörauf, S Kany, P Cavalli, J Omari, M Powerski, A Surov, I Marzi, AJ Nowak, B Relja
      Disease Markers, 2021;2021(0):6622701.  2021
    3. Transcorneal delivery of topically applied silver nanoparticles does not delay epithelial wound healing
      Authors: S Kim, BL Gates, M Chang, KE Pinkerton, L Van Winkle, CJ Murphy, BC Leonard, P Demokritou, SM Thomasy
      NanoImpact, 2021;24(0):100352.  2021
    4. Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro
      Authors: JA Hörauf, S Kany, A Janicova, B Xu, T Vrdoljak, R Sturm, IR Dunay, L Martin, B Relja
      Int J Mol Sci, 2020;21(9):.  2020
    5. A hidden risk: Survival and resuscitation of Escherichia coli O157:H7 in the viable but nonculturable state after boiling or microwaving
      Authors: Y Liu, T Kumblathan, GK Uppal, A Zhou, B Moe, SE Hrudey, XF Li
      Water Res., 2020;183(0):116102.  2020
    6. Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism
      Authors: J Lee, AE Yesilkanal, JP Wynne, C Frankenber, J Liu, J Yan, M Elbaz, DC Rabe, FD Rustandy, P Tiwari, EA Grossman, PC Hart, C Kang, SM Sanderson, J Andrade, DK Nomura, MG Bonini, JW Locasale, MR Rosner
      Nature, 2019;0(0):.  2019
    7. Combinatorial therapy of Zinc metallochaperones with mutant p53 reactivation and diminished copper binding
      Authors: S Zaman, X Yu, AF Bencivenga, AR Blanden, Y Liu, T Withers, B Na, AJ Blayney, J Gilleran, DA Boothman, SN Loh, SD Kimball, DR Carpizo
      Mol. Cancer Ther., 2019;0(0):.  2019
    8. Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells
      Authors: P Nguemgo Ko, GA Rezniczek, A Kochanneck, B Priesch-Gr, T Hero, IA Adamietz, H Bühler
      PLoS ONE, 2018;13(6):e0198508.  2018
    9. Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer
      Authors: MM Olcina, NG Balanis, RK Kim, BA Aksoy, J Kodysh, MJ Thompson, J Hammerbach, TG Graeber, AJ Giaccia
      Cell Rep, 2018;25(13):3721-3732.e6.  2018
    10. Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
      Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
      Aging Dis, 2017;8(2):203-214.  2017
    11. Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1? activation.
      Authors: Jonathan D Diedrich, Erandi Rajagurub, Mackenzie K Herroon, Gargi Mahapatra, Maik Hüttemann, Izabela Podgorski
      Oncotarget, 2016;0(0):1949-2553.  2016
    12. Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
      Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
      J Leukoc Biol, 2014;96(3):365-75.  2014
    13. Prion protein interaction with soil humic substances: environmental implications.
      Authors: Giachin, Gabriele, Narkiewicz, Joanna, Scaini, Denis, Ngoc, Ai Tran, Margon, Alja, Sequi, Paolo, Leita, Liviana, Legname, Giuseppe
      PLoS ONE, 2014;9(6):e100016.  2014

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