CALHM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92818

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CALHM1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 202-346 of human CALHM1 (NP_001001412.3). VRSVRPCFTQAAFLKSKYWSHYIDIERKLFDETCTEHAKAFAKVCIQQFFEAMNHDLELGHTHGTLATAPASAAAPTTPDGAEEEREKLRGITDQGTMNRLLTSWHKCKPPLRLGQEEPPLMGNGWAGGGPRPPRKEVATYFSKV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CALHM1 Antibody - BSA Free

Western Blot: CALHM1 AntibodyBSA Free [NBP2-92818]

Western Blot: CALHM1 AntibodyBSA Free [NBP2-92818]

Western Blot: CALHM1 Antibody [NBP2-92818] - Analysis of extracts of various cell lines, using CALHM1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: CALHM1 Antibody - BSA Free [NBP2-92818]

Immunohistochemistry-Paraffin: CALHM1 Antibody - BSA Free [NBP2-92818]

Immunohistochemistry-Paraffin: CALHM1 Antibody [NBP2-92818] - Immunohistochemistry of paraffin-embedded rat brain using CALHM1 antibody (NBP2-92818) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for CALHM1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CALHM1

CALHM1 controls cytosolic calcium permeability and cytosolic calcium concentration. It controls amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a calcium dependent manner. However, no association between CALHM1 and Alzheimer's risk is unclear. Member of the FAM26 family of proteins. CALHM1 is predominantly expressed in the adult brain. It has also been detected in retinoic acid differentiated SH-SY5Y cells. Also colocalizes with HSPA5 at the endoplasmic reticulum.

Alternate Names

calcium homeostasis modulator 1, FAM26Ccalcium homeostasis modulator protein 1, family with sequence similarity 26, member C, MGC39514, MGC39617, Protein FAM26C

Gene Symbol

CALHM1

Additional CALHM1 Products

Product Documents for CALHM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CALHM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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