Calmodulin Antibody (SJ16-09)

Novus Biologicals | Catalog # NBP2-67413

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SJ16-09 expressed in HEK293
View all Calmodulin 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Calmodulin aa 100-149 / 149. (SwissProt: P0DP23 Human; SwissProt: P0DP24 Human; SwissProt: P0DP25 Human)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calmodulin Antibody (SJ16-09)

Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413]

Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.

Applications for Calmodulin Antibody (SJ16-09)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calmodulin 1

Calmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).

Alternate Names

CALM1, CALML2, CaM, CAM1, CAMI, CPVT4, DD132

Gene Symbol

CALM1

Additional Calmodulin 1 Products

Product Documents for Calmodulin Antibody (SJ16-09)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Calmodulin Antibody (SJ16-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calmodulin Antibody (SJ16-09)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Calmodulin Antibody (SJ16-09)

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  • Q: I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K

    A:

    Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.

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