Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70334

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G1

Format

Azide and BSA Free
View all Calpain 2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

Western Blot: Calpain 2 Antibody (OTI3G1)Azide and BSA Free [NBP2-70334]

Western Blot: Calpain 2 Antibody (OTI3G1)Azide and BSA Free [NBP2-70334]

Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334]

Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calpain 2

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Alternate Names

Calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, Calpain large polypeptide L2, Calpain M-type, calpain, large polypeptide L2, calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2CANP2, CANPml, EC 3.4.22, EC 3.4.22.53, FLJ39928, M-calpain, mCANP, Millimolar-calpain

Gene Symbol

CAPN2

Additional Calpain 2 Products

Product Documents for Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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