Calpain 3 Antibody (1B2) - Azide and BSA Free

Novus Biologicals | Catalog # H00000825-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B2

Format

Azide and BSA Free
View all Calpain 3 Primary Antibodies »

Product Specifications

Immunogen

CAPN3 (AAH03169.1, 210 a.a. ~ 309 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Specificity

Reacts with calpain 3, (p94).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Calpain 3 Antibody (1B2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody (1B2) [H00000825-M01]

Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody (1B2) [H00000825-M01]

Immunocytochemistry/Immunofluorescence: Calpain 3 Antibody (1B2) [H00000825-M01] - Analysis of monoclonal antibody to CAPN3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Calpain 3 Antibody (1B2) [H00000825-M01] - Detection limit for recombinant GST tagged CAPN3 is 0.3 ng/ml as a capture antibody.

Applications for Calpain 3 Antibody (1B2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain 3

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq]

Alternate Names

calpain 3, (p94), Calpain L3, Calpain p94, calpain, large polypeptide L3, calpain-3, CANP 3, CANP3MGC4403, CANPL3, EC 3.4.22, EC 3.4.22.54, LGMD2, LGMD2A, MGC10767, MGC11121, MGC14344, Muscle-specific calcium-activated neutral protease 3, muscle-specific calcium-activated neutral protease 3 large subunit, NCL1, nCL-1large [catalytic] subunit, New calpain 1

Entrez Gene IDs

825 (Human)

Gene Symbol

CAPN3

UniProt

AAH03169 (Human)

Additional Calpain 3 Products

Product Documents for Calpain 3 Antibody (1B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calpain 3 Antibody (1B2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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