Calreticulin-2/CALR3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-33524
![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0006.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKGVWLHRKMKNTDYLTQYDLSEF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Calreticulin-2/CALR3 Antibody - BSA Free
![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0007.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human colon, kidney, liver and testis using Anti-CALR3 antibody NBP2-33524 (A) shows similar protein distribution across tissues to independent antibody NBP2-33390 (B).![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0011.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0011.jpg![Western Blot: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Western-Blot-NBP2-33524-img0002.jpg "Western Blot: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Western Blot: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Western Blot: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0010.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human colon.![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0004.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human endometrium shows low expression as expected.![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0005.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human testis shows high expression.![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0008.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human kidney.![Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]](https://resources.rndsystems.com/images/products/Calreticulin-2-CALR3-Antibody-Immunohistochemistry-Paraffin-NBP2-33524-img0009.jpg "Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]")
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human liver.
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33524] -
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Immunohistochemical staining of CT antigens in lung & breast cancer tumors.Examples of lung & breast cancer tumors positive for MAGE-A & negative for ADAM2, CALR3 & SAGE1 (all pictures magnification x20). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0134967), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33524] -
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Immunohistochemical staining of CT antigens in normal tissues.Among 22 normal tissues (see materials & methods), ADAM2, CALR3, SAGE1 & MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 & MAGE-A were found in spermatogonia, while ADAM2 & CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in plancenta (all pictures magnification x20). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0134967), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Calreticulin-2/CALR3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calreticulin-2
Additional Calreticulin-2 Products
Product Documents for Calreticulin-2/CALR3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Calreticulin-2/CALR3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Calreticulin-2/CALR3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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