CaM Kinase II Antibody (9P2E2)

Novus Biologicals | Catalog # NBP3-15256

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9P2E2 expressed in HEK293
View all CaM Kinase II Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CaM Kinase II (NP_741960.1). GVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II Antibody (9P2E2)

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded rat brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded human brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
CaM Kinase II Antibody (9P2E2)

Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] -

Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaM Kinase II Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for CaM Kinase II Antibody (9P2E2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II

Calmodulin kinase II (CaMKII) is an 8 - 12 subunit multimeric calcium/calmodulin dependent Ser/Thr protein kinase. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes (alpha, beta, gamma, delta), each of which contains a catalytic, autoregulatory, and subunit-association domain.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II

Alternate Names

CAMK2, CAMK2B

Gene Symbol

CAMK2B

Additional CaM Kinase II Products

Product Documents for CaM Kinase II Antibody (9P2E2)

Certificate of Analysis

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Product Specific Notices for CaM Kinase II Antibody (9P2E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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