CaMKK alpha Antibody (1F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00084254-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F6

Format

Azide and BSA Free
View all CaMKK alpha Primary Antibodies »

Product Specifications

Immunogen

CAMKK1 (AAH31647, 1 a.a. ~ 520 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEGPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLV

Reactivity Notes

Other species not tested.

Specificity

CAMKK1 - calcium/calmodulin-dependent protein kinase kinase 1, alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CaMKK alpha Antibody (1F6) - Azide and BSA Free

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 monoclonal antibody (M01), clone 1F6 Analysis of CAMKK1 expression in Jurkat.
Immunohistochemistry-Paraffin: CaMKK alpha Antibody (1F6) [H00084254-M01]

Immunohistochemistry-Paraffin: CaMKK alpha Antibody (1F6) [H00084254-M01]

Immunohistochemistry-Paraffin: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.
Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 over-expressed 293 cell line. Lane 2 cotransfected with CAMKK1 Validated Chimera RNAi or non-transfected control. Blot probed with H00084254-M01. GAPDH (36.1 kDa) used as loading control.
Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01]

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.Lane 1: CAMKK1 transfected lysate(57.31 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CaMKK alpha Antibody (1F6) [H00084254-M01] - Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.

Applications for CaMKK alpha Antibody (1F6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CaMKK alpha

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.

Long Name

Calcium/Calmodulin-dependent Protein Kinase Kinase alpha

Alternate Names

CaM-kinase IV kinase, CaMKK1

Entrez Gene IDs

84254 (Human)

Gene Symbol

CAMKK1

UniProt

AAH31647 (Human)

Additional CaMKK alpha Products

Product Documents for CaMKK alpha Antibody (1F6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CaMKK alpha Antibody (1F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail