CaMKK alpha Antibody

Novus Biologicals | Catalog # NBP1-42683

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CaMKK alpha Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 455 and 505 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaMKK alpha Antibody

Western Blot: CaMKK alpha Antibody [NBP1-42683]

Western Blot: CaMKK alpha Antibody [NBP1-42683]

Western Blot: CaMKK alpha Antibody [NBP1-42683] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CaMKK alpha antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CaMKK alpha was also immunoprecipitated by rabbit anti-CaMKK alpha antibody NBP1-42682, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683]

Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683]

Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683] - Section of human lung carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- CaMKK alpha used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for CaMKK alpha Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CaMKK alpha

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Calcium/Calmodulin-dependent Protein Kinase Kinase alpha

Alternate Names

CaM-kinase IV kinase, CaMKK1

Entrez Gene IDs

84254 (Human)

Gene Symbol

CAMKK1

Additional CaMKK alpha Products

Product Documents for CaMKK alpha Antibody

Certificate of Analysis

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Product Specific Notices for CaMKK alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CaMKK alpha Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail