Canine CD34 PE‑conjugated Antibody

R&D Systems | Catalog # FAB3346P

R&D Systems
Discontinued Product
FAB3346P has been discontinued. View all CD34 products.

Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Rat, Canine

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H6
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Specificity

Detects canine CD34 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine CD34 PE‑conjugated Antibody

Detection of CD34 antibody in CLL-1390 Canine Cell Line antibody by Flow Cytometry.

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB3346P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Canine CD34 by Flow Cytometry

Detection of Canine CD34 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine CD34 by Flow Cytometry

Detection of Canine CD34 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Canine CD34 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CLL‑1390 canine leukocytic round cell neoplasia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using FAB3346P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

Additional CD34 Products

Product Documents for Canine CD34 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Canine CD34 PE‑conjugated Antibody

For research use only

Citations for Canine CD34 PE‑conjugated Antibody

Customer Reviews for Canine CD34 PE‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: A G
    Application: Flow Cytometry
    Sample Tested: Whole blood lymphocytes
    Species: Canine
    Verified Customer | Posted 01/31/2017
    Immunophenotyping in dogs
    Cells are lysed and then incubated with antibody for 30mins. The washed cells are then read on BD FACS Cailbur
    Canine CD34 PE‑conjugated Antibody FAB3346P
  • Name: A G
    Application: Flow Cytometry
    Sample Tested: Whole blood cells
    Species: Other
    Verified Customer | Posted 03/22/2016
    Canine IP
    Canine CD34 PE‑conjugated Antibody FAB3346P

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