Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD4, recombinant mouse CD4, and recombinant feline CD4 is observed.
Monoclonal Mouse IgG2B Clone # 296712
Protein A or G purified from hybridoma culture supernatant
Detection of CD4 in Lymphocytes in Canine PBMCs by Flow Cytometry.
Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2410A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD4 is a 56-58 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on a variety of cell types including T helper cells, follicular helper cells, neurons, T regulatory cells, microglia, and monocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. Over aa 25-401, canine CD4 shares 56% amino acid identity with human CD4.
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