![Western Blot: CapG AntibodyBSA Free [NBP2-92955]](https://resources.rndsystems.com/images/products/CapG-Antibody-Western-Blot-NBP2-92955-img0005.jpg "Western Blot: CapG AntibodyBSA Free [NBP2-92955]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2). MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Knockout (KO) Validated Rabbit CapG Antibody - BSA Free (NBP2-92955) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CapG Antibody - BSA Free
Western Blot: CapG AntibodyBSA Free [NBP2-92955]
Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts of various cell lines, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.![Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]](https://resources.rndsystems.com/images/products/CapG-Antibody-Immunohistochemistry-Paraffin-NBP2-92955-img0002.jpg "Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]")
Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]
Immunohistochemistry-Paraffin: CapG Antibody [NBP2-92955] - Paraffin-embedded human lung cancer using CapG.![Western Blot: CapG AntibodyBSA Free [NBP2-92955]](https://resources.rndsystems.com/images/products/CapG-Antibody-Western-Blot-NBP2-92955-img0003.jpg "Western Blot: CapG AntibodyBSA Free [NBP2-92955]")
Western Blot: CapG AntibodyBSA Free [NBP2-92955]
Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.![Western Blot: CapG AntibodyBSA Free [NBP2-92955]](https://resources.rndsystems.com/images/products/CapG-Antibody-Western-Blot-NBP2-92955-img0004.jpg "Western Blot: CapG AntibodyBSA Free [NBP2-92955]")
Western Blot: CapG AntibodyBSA Free [NBP2-92955]
Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts of various cell lines, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.![Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]](https://resources.rndsystems.com/images/products/CapG-Antibody-Immunohistochemistry-Paraffin-NBP2-92955-img0001.jpg "Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]")
Immunohistochemistry-Paraffin: CapG Antibody - BSA Free [NBP2-92955]
Immunohistochemistry-Paraffin: CapG Antibody [NBP2-92955] - Paraffin-embedded human liver cancer using CapG.Applications for CapG Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CapG
Long Name
Capping Protein, Gelsolin-like
Alternate Names
AFCP
Gene Symbol
CAPG
Additional CapG Products
Product Documents for CapG Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CapG Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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