CAPZB Antibody

Novus Biologicals | Catalog # NB100-814

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CAPZB Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence NDLVEALKRKQQC corresponding to C-Terminus according to NP_004921.1.

Specificity

This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CAPZB Antibody

Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814] - (1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane).
Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814] - Analysis of of Jurkat (A), HeLa (B) and NIH-3T3 (C) lysates (35ug protein in RIPA buffer).
Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814]

Western Blot: CAPZB Antibody [NB100-814] - Analysis of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer).

Applications for CAPZB Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in Jurkat, HeLa and NIH-3T3 lysates and approx. 28 kDa band observed in mouse, rat and porcine heart lysates (calculated MW of 30.6 kDa band according to human NP_004921.1 and 31.3 kDa band according to mouse NP_001032850.1, and 30.6 kDa band according to rat NP_001005903.1 and porcine NP_001106915.1].

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CAPZB

CAPZB is involved in the regulation of growth of the actin filament by capping the barbed end of growing actin filaments

Alternate Names

Cap Z, CAPB, CAPPB, capping protein (actin filament) muscle Z-line, beta, CAPZ, CapZ beta, F-actin capping protein beta subunit, F-actin-capping protein subunit beta, MGC104401, MGC129749, MGC129750

Entrez Gene IDs

832 (Human); 12345 (Mouse)

Gene Symbol

CAPZB

UniProt

NP_004921.1 (Human)

Additional CAPZB Products

Product Documents for CAPZB Antibody

Certificate of Analysis

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Product Specific Notices for CAPZB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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