Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00009469-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D3

Format

Azide and BSA Free
View all Carbohydrate Sulfotransferase 3/CHST3 Primary Antibodies »

Product Specifications

Immunogen

CHST3 (NP_004264, 312 a.a. ~ 411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VAFAGKYKTWKKWLDDEGQDGLREEEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVEDWIQKNTQAAHDG

Reactivity Notes

This product is reactive against Human.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images

Western Blot: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02]

Western Blot: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02]

Western Blot: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02] - CHST3 monoclonal antibody (M02), clone 1D3. Analysis of CHST3 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02]

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02]

Immunocytochemistry/Immunofluorescence: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02] - Analysis of monoclonal antibody to CHST3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Carbohydrate Sulfotransferase 3/CHST3 Antibody (1D3) [H00009469-M02] - Detection limit for recombinant GST tagged CHST3 is 0.3 ng/ml as a capture antibody.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carbohydrate Sulfotransferase 3/CHST3

The Carbohydrate sulfotransferase 3 gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal

Alternate Names

C6ST1, CHST3, GST-0

Gene Symbol

CHST3

UniProt

NP_004264 (Human)

Additional Carbohydrate Sulfotransferase 3/CHST3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail