Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
Novus Biologicals | Catalog # NBP1-47704
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2A3
Loading...
Product Specifications
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-419 of human CPA-1
Specificity
This antibody is specific for Homo sapiens carboxypeptidase A1 (pancreatic) (CPA1).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
![Western Blot: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Western-Blot-NBP1-47704-img0022.jpg "Western Blot: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Western Blot: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Western Blot: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Carboxypeptidase A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Carboxypeptidase A.![Immunocytochemistry/ Immunofluorescence: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunocytochemistry-Immunofluorescence-NBP1-47704-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunocytochemistry/ Immunofluorescence: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunocytochemistry/Immunofluorescence: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY Carboxypeptidase A.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0021.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded liver using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0013.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0014.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Adenocarcinoma of ovary using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0015.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded breast using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0016.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of kidney using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0017.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of liver using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0018.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0019.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded endometrium using anti-Carboxypeptidase A mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]](https://resources.rndsystems.com/images/products/Carboxypeptidase-A1-CPA1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP1-47704-img0020.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]")
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (OTI2A3) [NBP1-47704]
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded kidney using anti-Carboxypeptidase A mouse monoclonal antibody.Applications for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Reviewed Applications
Read 1 review rated 5 using NBP1-47704 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Carboxypeptidase A1/CPA1
Additional Carboxypeptidase A1/CPA1 Products
Product Documents for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
Powered by Bioz
Customer Reviews for Carboxypeptidase A1/CPA1 Antibody (OTI2A3) (1)
5 out of 5
1 Customer Rating
Have you used Carboxypeptidase A1/CPA1 Antibody (OTI2A3)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Immunohistochemistry-ParaffinSample Tested: human pancreasSpecies: HumanVerified Customer | Posted 11/16/2016CPA1 expression (magenta) in human pancreatic tissue. Tissue was paraffin-embedded and no permeabilization or antigen retrieval was required.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: I would like to know if anybody has tried to use this anti-Carboxypeptidase A antibody (NBP1-47704) for immunohistochemistry in mouse tissues?
A: This antibody has never been tested by our lab or reported to us by a customer for staining of mouse tissues. The most likely reason for this isn't necessarily the sequence homology, but due to the fact that this is a mouse monoclonal antibody staining on tissues of the same host can be problematic because non-specific binding of the secondary could cause high background and make interpretation of your samples difficult.
Loading...