Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001360-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D5

Format

Azide and BSA Free
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Product Specifications

Immunogen

CPB1 (AAH15338, 14 a.a. ~ 417 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAHHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY

Specificity

carboxypeptidase B1 (tissue)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of CPB1 expression in transfected 293T cell line by CPB1 monoclonal antibody (M01), clone 4D5.Lane 1: CPB1 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of monoclonal antibody to CPB1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Detection against Immunogen (70.55 KDa)
Immunoprecipitation: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Immunoprecipitation: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01]

Immunoprecipitation: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of CPB1 transfected lysate using anti-CPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPB1 MaxPab rabbit polyclonal antibody.

Applications for Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carboxypeptidase B1/CPB1

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Alternate Names

CPB1, PASP, PCPB

Entrez Gene IDs

1360 (Human)

Gene Symbol

CPB1

OMIM

114852 (Human)

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carboxypeptidase B1/CPB1 Antibody (4D5) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

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