Carboxypeptidase B1/CPB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15698

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carboxypeptidase B1/CPB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase B1/CPB1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (86%), Porcine (85%), Bovine (83%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carboxypeptidase B1/CPB1 Antibody - BSA Free

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Paraffin-embedded rat pancreas. Carboxypeptidase B antibody [N3C3] diluted at 1:1000.
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698]

Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Paraffin-embedded mouse pancreas. Carboxypeptidase B antibody [N3C3] diluted at 1:1000.

Applications for Carboxypeptidase B1/CPB1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carboxypeptidase B1/CPB1

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]

Alternate Names

CPB1, PASP, PCPB

Gene Symbol

CPB1

UniProt

P15086

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Carboxypeptidase B1/CPB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carboxypeptidase B1/CPB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carboxypeptidase B1/CPB1 Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

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