CARD10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33042

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CARD10/CARMA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CARD10. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD10 Antibody - BSA Free

Western Blot: CARD10 Antibody [NBP1-33042]

Western Blot: CARD10 Antibody [NBP1-33042]

Western Blot: CARD10 Antibody [NBP1-33042] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042]

Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042]

Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042] - Human breast carcinoma. CARD10 antibody [N2C1], Internal diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
CARD10 Antibody

Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042] -

Immunohistochemistry-Paraffin: CARD10 Antibody [NBP1-33042] -Mouse kidney.CARD10 stained by CARD10 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CARD10 Antibody

Western Blot: CARD10 Antibody [NBP1-33042] -

Western Blot: CARD10 Antibody [NBP1-33042] - Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.
CARD10 Antibody

Western Blot: CARD10 Antibody [NBP1-33042] -

Western Blot: CARD10 Antibody [NBP1-33042] -Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CARD10 Antibody

Western Blot: CARD10 Antibody [NBP1-33042] -

Western Blot: CARD10 Antibody [NBP1-33042] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
CARD10 Antibody

Western Blot: CARD10 Antibody [NBP1-33042] -

Western Blot: CARD10 Antibody [NBP1-33042] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CARD10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 21078624)

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CARD10/CARMA3

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARDs induce nuclear factor kappa-B (NFKB; MIM 164011) activity through the IKK (e.g., IKBKB; MIM 603258) complex. CARD9 (MIM 607212), CARD10, CARD11 (MIM 607210), and CARD14 (MIM 607211) interact with BCL10 (MIM 603517) and are involved in NFKB signaling complexes. Except for CARD9, these CARD proteins are members of the membrane-associated guanylate kinase (MAGUK) family.

Long Name

Caspase Recruitment Domain-containing Protein 10

Alternate Names

BIMP1, CARMA3

Gene Symbol

CARD10

Additional CARD10/CARMA3 Products

Product Documents for CARD10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CARD10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for CARD10 Antibody - BSA Free

Customer Reviews for CARD10 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review CARD10 Antibody - BSA Free and earn rewards!

Have you used CARD10 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies