CARD6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15704

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CARD6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CARD6. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD6 Antibody - BSA Free

Immunohistochemistry: CARD6 Antibody [NBP2-15704]

Immunohistochemistry: CARD6 Antibody [NBP2-15704]

CARD6-Antibody-Immunohistochemistry-NBP2-15704-img0005.jpg
Western Blot: CARD6 Antibody [NBP2-15704]

Western Blot: CARD6 Antibody [NBP2-15704]

Western Blot: CARD6 Antibody [NBP2-15704] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704]

Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704]

Immunocytochemistry/Immunofluorescence: CARD6 Antibody [NBP2-15704] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CARD6 Antibody [NBP2-15704]

Immunohistochemistry-Paraffin: CARD6 Antibody [NBP2-15704]

Immunohistochemistry-Paraffin: CARD6 Antibody [NBP2-15704] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
CARD6 Antibody

Immunohistochemistry-Paraffin: CARD6 Antibody [NBP2-15704] -

Induced expression of NOD2, CARD6, PYCARD, & IFI16 in psoriasis lesions.(a) Immunohistochemistry shows stronger NOD2, CARD6, PYCARD, & IFI16 staining in PL (upper) than in controls (lower). PN (middle) & C are almost negative for NOD2 & CARD6. IFI16 is predominantly in nuclei of PL & PN. In controls IFI16 expression is weak & cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL & PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 μm. (b) IEM of PL shows PYCARD clusters in cytoplasm. (c) Immunofluorescence of keratinocytes colocalizes CARD6 with a mitochondrial MTCO2. (d) IEM of psoriatioc lesional samples also localized CARD6 in the mitochondria (arrow head). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26976200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CARD6 Antibody

Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704] -

Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704] - Induced expression of NOD2, CARD6, PYCARD, & IFI16 in psoriasis lesions.(a) Immunohistochemistry shows stronger NOD2, CARD6, PYCARD, & IFI16 staining in PL (upper) than in controls (lower). PN (middle) & C are almost negative for NOD2 & CARD6. IFI16 is predominantly in nuclei of PL & PN. In controls IFI16 expression is weak & cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL & PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 μm. (b) IEM of PL shows PYCARD clusters in cytoplasm. (c) Immunofluorescence of keratinocytes colocalizes CARD6 with a mitochondrial MTCO2. (d) IEM of psoriatioc lesional samples also localized CARD6 in the mitochondria (arrow head). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26976200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CARD6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
Electron microscopy usage reported in scientific literature (PMID: 26976200).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CARD6

This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq]

Long Name

Caspase Recruitment Domain-containing Protein 6

Alternate Names

CINCIN1

Gene Symbol

CARD6

UniProt

Q9BX69 (Human)

Additional CARD6 Products

Product Documents for CARD6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CARD6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cysteine Proteases and Regulators

Citations for CARD6 Antibody - BSA Free

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Protocols

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