Casein Kinase 2 alpha Antibody

Novus Biologicals | Catalog # NB100-377

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative), Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Casein Kinase 2 alpha Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Casein Kinase 2 alpha Antibody

Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for Casein Kinase 2 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 -1:2000

Immunohistochemistry-Paraffin

1:500 -1:2000

Western Blot

1:2000 - 1:10000
Application Notes
By WB, this antibody recognizes a band at ~42 kDa, representing CKII alpha. Not recommended for immunoprecipitation. *NB 100-378 is recommended for IP. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-buffered Saline, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Casein Kinase 2 alpha

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Alternate Names

CK2A1, CSNK2A1

Entrez Gene IDs

1457 (Human)

Gene Symbol

CSNK2A1

UniProt

NP_001886.1 (Human)

Additional Casein Kinase 2 alpha Products

Product Documents for Casein Kinase 2 alpha Antibody

Certificate of Analysis

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Product Specific Notices for Casein Kinase 2 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
Notch Pathway

Citations for Casein Kinase 2 alpha Antibody

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail