CASK Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-41181

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CASK Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK.

Specificity

At least six alternatively spliced transcript variants have been observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CASK Antibody - BSA Free

Western Blot: CASK AntibodyBSA Free [NBP2-41181]

Western Blot: CASK AntibodyBSA Free [NBP2-41181]

Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Simple Western: CASK AntibodyBSA Free [NBP2-41181]

Simple Western: CASK AntibodyBSA Free [NBP2-41181]

Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.
CASK Antibody - BSA Free

Simple Western: CASK Antibody - BSA Free [NBP2-41181] -

Simple Western: CASK Antibody - BSA Free [NBP2-41181] - Presynaptic effect is limited to inhibitory VGAT presynaptic marker.a Deconvolution of cell-type populations underlying bulk RNA-sequence samples from all cell lines. b Quantification of VGlut & Homer-1 particle size (n = 4 per cell line). c Representative confocal microscopy images of neurons differentiated for 28 days & immunostained for inhibitory VGAT (green), Homer-1 (red), & Hoechst (blue). Scale = 10 µm. d Quantification of VGAT & Homer-1 particle size (n = 3 per cell line). Statistical differences between cell lines were calculated using ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01, ***p < 0.001. Asterisks are color-coded according to case cell lines. e In vivo concentration of GABA in the DLPFC, MFC, & putamen of ASDCASK_SS & his cotwin in relation to a typical developed control group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32929080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CASK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CASK Antibody - BSA Free [NBP2-41181] -

Immunocytochemistry/ Immunofluorescence: CASK Antibody - BSA Free [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.

Applications for CASK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

1 ug/mL
Application Notes
Simple Western reported in a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in Differentiating human neuroepithelial stem cells, separated by Size, antibody dilution of 1:500 and 1:1000, apparent MW was 116, 38 kDa

Reviewed Applications

Read 1 review rated 5 using NBP2-41181 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CASK

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Alternate Names

Calcium/calmodulin-dependent serine protein kinase, calcium/calmodulin-dependent serine protein kinase (MAGUK family), calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase, CAMGUK, CMG, EC 2.7.11, EC 2.7.11.1, FGS4CAGH39, FLJ22219, hCASK, LIN2FLJ31914, MICPCH, peripheral plasma membrane protein CASK, Protein lin-2 homolog, TNRC8, trinucleotide repeat containing 8

Gene Symbol

CASK

Additional CASK Products

Product Documents for CASK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CASK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CASK Antibody - BSA Free

Customer Reviews for CASK Antibody - BSA Free (1)

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  • CASK Antibody
    Name: Martin Becker
    Application: Simple Western
    Sample Tested: Differentiated neuron progenitor cells
    Species: Human
    Verified Customer | Posted 03/13/2017
    0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa.
    Protein lysates have been generated using detergent free lysis buffer (TrisHCl, NaCl, EDTA, EGTA) and 5 seconds of sonication.
    CASK Antibody - BSA Free NBP2-41181

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