Caspase-14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33906

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-14 Antibody - BSA Free

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906] - Analysis in human skin and skeletal muscle tissues using NBP2-33906 antibody. Corresponding Caspase-14 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Caspase-14 Antibody [NBP2-33906]

Immunocytochemistry/ Immunofluorescence: Caspase-14 Antibody [NBP2-33906]

Immunocytochemistry/Immunofluorescence: Caspase-14 Antibody [NBP2-33906] - Staining of human cell line hTCEpi shows localization to nucleus, cytosol & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906] - Staining of human prostate shows no positivity in glandular cells.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906] - Staining of human skin shows strong cytoplasmic and nuclear positivity in keratinocytes.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NBP2-33906] - Staining of human testis shows no positivity in cells in seminiferous ducts.

Applications for Caspase-14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-14

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-14 activation has been implicated in keratinocyte senescence which leads to the cornified cell layer, suggesting a role for caspase-14 in epithelial cell differentiation (Pistritto et al. 2002). Terminal differentiation of keratinocytes has some features of classical apoptosis including DNA fragmentation, nuclear condensation, and induction of cross-linking transglutaminases. Tumor-specific alterations in caspase-14 expression have been found in epithelial malignancies, suggesting that caspase-14 plays a role in epithelial cell transformation. The caspase-14 IMG-5713 antibody recognizes the proform of caspase-14 (approx. 28-32 kDa), and the large (approx. 14-21 kDa) and small (approx. 10-11 kDa) of active/cleaved caspase-14.

Alternate Names

CASP14, Caspase14

Gene Symbol

CASP14

UniProt

P31944 (Human)

Additional Caspase-14 Products

Product Documents for Caspase-14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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