Caspase-8 Antibody - BSA Free

A: I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.

A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

A:

The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

A: I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.

A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

A:

The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

A: I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.

A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

A:

The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.