Caspase-9 Antibody (9M6N2)

Novus Biologicals | Catalog # NBP3-15590

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9M6N2 expressed in HEK293
View all Caspase-9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2). VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-9 Antibody (9M6N2)

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of C6 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Caspase-9 Antibody (9M6N2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody (9M6N2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caspase-9 Antibody (9M6N2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-9 Antibody (9M6N2)

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  • Q: Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?

    A: After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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