Caspr2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31358

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspr2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspr2. The exact sequence is proprietary.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspr2 Antibody - BSA Free

Immunohistochemistry-Frozen: Caspr2 Antibody [NBP1-31358]

Immunohistochemistry-Frozen: Caspr2 Antibody [NBP1-31358]

Immunohistochemistry-Frozen: Caspr2 Antibody [NBP1-31358] - Caspr2 antibody [N2N3] detects Caspr2n proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Caspr2 antibody [N2N3] diluted at 1:250. Blue: DAPI.
Caspr2 Antibody

Western Blot: Caspr2 Antibody [NBP1-31358] -

Western Blot: Caspr2 Antibody [NBP1-31358] - Caspr2 antibody detects Caspr2 protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Caspr2 antibody (NBP1-31358) diluted by 1:5000
Caspr2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caspr2 Antibody [NBP1-31358] -

Caspr2 antibody [N2N3] detects Caspr2 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (NBP1-31358) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Caspr2 Antibody - BSA Free

Western Blot: Caspr2 Antibody [NBP1-31358] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Caspr2 antibody [N2N3] (NBP1-31358) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Caspr2 Antibody - BSA Free

Western Blot: Caspr2 Antibody [NBP1-31358] -

Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Caspr2 antibody [N2N3] (NBP1-31358) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Caspr2 Antibody - BSA Free

Immunohistochemistry-Frozen: Caspr2 Antibody [NBP1-31358] -

Caspr2 antibody [N2N3] detects Caspr2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (NBP1-31358) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Caspr2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caspr2

This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and associated with potassium channels. It may play a role in the local differentiation of the axon into distinct functional subdomains. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It may represent a positional candidate gene for the DFNB13 form of nonsyndromic deafness.

Long Name

Contactin Associated Protein-like 2

Alternate Names

CDFE, CNTNAP2, NRXN4

Gene Symbol

CNTNAP2

Additional Caspr2 Products

Product Documents for Caspr2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspr2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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