Caspr2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88182

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CNKDVGAFFEEGMWLRYNFQAPATNARDSSSRVDNAPDQQNSHPDLAQEEIRFSFSTTKAPCILLYISSFTTDFLAVLVKPTGSLQIRYNLGGTREPYNIDVDHRNMANGQPHSVNITRHEKTIFLKLDHYPSVSYHLPSSS

Reactivity Notes

Mouse (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspr2 Antibody - BSA Free

Caspr2 Antibody - BSA Free Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Analysis in human cerebral cortex and pancreas tissues using HPA002739 antibody. Corresponding CNTNAP2 RNA-seq data are presented for the same tissues.
Caspr2 Antibody - BSA Free Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: Caspr2 Antibody [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody [NBP1-88182] - Staining of human pancreas shows low expression as expected.
Caspr2 Antibody - BSA Free Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Staining of human skin shows no positivity in squamous epithelial cells as expected.
Caspr2 Antibody - BSA Free Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Immunohistochemistry-Paraffin: Caspr2 Antibody - BSA Free [NBP1-88182]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Western Blot: Caspr2 Antibody [NBP1-88182]

Western Blot: Caspr2 Antibody [NBP1-88182]

Western Blot: Caspr2 Antibody [NBP1-88182] - Analysis in control (vector only transfected HEK293T lysate) and CNTNAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for Caspr2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-88182 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspr2

Caspr2 encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and associated with potassium channels. It may play a role in the local differentiation of the axon into distinct functional subdomains. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It may represent a positional candidate gene for the DFNB13 form of nonsyndromic deafness.

Long Name

Contactin Associated Protein-like 2

Alternate Names

CDFE, CNTNAP2, NRXN4

Gene Symbol

CNTNAP2

Additional Caspr2 Products

Product Documents for Caspr2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Caspr2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspr2 Antibody - BSA Free

Customer Reviews for Caspr2 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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4 Stars
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Filter By:
  • Name: YONGJIE LAI
    Application: Western Blot
    Sample Tested: Caspr2 plasmid transfected HEK293T cell lysate
    Species: Human
    Verified Customer | Posted 09/24/2014

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