Cathepsin A/Lysosomal Carboxypeptidase A Antibody (RM0160-4A12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-12071

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0160-4A12

Format

Azide and BSA Free
View all Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies »

Product Specifications

Immunogen

Mouse recombinant Cathepsin A/Lysosomal Carboxypeptidase A

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Applications

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:400

Western Blot

1:500-1:1000
Application Notes
This antibody may also work in ELISA. In western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Gene Symbol

CTSA

UniProt

P16675

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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  • Q: How should I reconstitute this antibody?

    A: You should reconstitute this antibody in 200 uL sterile PBS.

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