Cathepsin H Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90273

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cathepsin H Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPSQAFEYILYNKGIMGEDTYPYQGKDGYCKFQPGKAIGFVKDVANITIYDEEAMVEAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVNHAVLAVGYGEKNGIPYWIVKNSWGPQWGMNGYFL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin H Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cathepsin H Antibody [NBP1-90273]

Immunocytochemistry/ Immunofluorescence: Cathepsin H Antibody [NBP1-90273]

Immunocytochemistry/Immunofluorescence: Cathepsin H Antibody [NBP1-90273] - Staining of human cell line U-2 OS shows localization to vesicles.
Cathepsin H Antibody - BSA Free Immunohistochemistry: Cathepsin H Antibody - BSA Free [NBP1-90273]

Immunohistochemistry: Cathepsin H Antibody - BSA Free [NBP1-90273]

Analysis in human lung and skeletal muscle tissues using Anti-CTSH antibody. Corresponding CTSH RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273]

Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273]

Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273]

Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273]

Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273] - Staining of human skeletal muscle shows low expression as expected.

Applications for Cathepsin H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin H

Cathepsin H is encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

CTSH

Gene Symbol

CTSH

Additional Cathepsin H Products

Product Documents for Cathepsin H Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cathepsin H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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