Caveolin-2 Antibody (5E9E2) - BSA Free

Novus Biologicals | Catalog # NBP2-52431

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5E9E2

Format

BSA Free
View all Caveolin-2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Caveolin-2 (AA: 1-86) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caveolin-2 Antibody (5E9E2) - BSA Free

Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mouse mAb against A549 (1), 3T3-L1 (2), A431 (3) cell lysate.
Immunohistochemistry-Paraffin: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Immunohistochemistry-Paraffin: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Immunohistochemistry-Paraffin: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.
Flow Cytometry: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Flow Cytometry: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Flow Cytometry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of A549 cells using CAV2 mouse mAb (green) and negative control (red).
Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against HEK293 (1) and CAV2 (AA: 1-86)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2)BSA Free [NBP2-52431]

Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against human CAV2 (AA: 1-86) recombinant protein. (Expected MW is 35.9 kDa)
Immunohistochemistry: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Immunohistochemistry: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

Immunohistochemistry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.
ELISA: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

ELISA: Caveolin-2 Antibody (5E9E2) - BSA Free [NBP2-52431]

ELISA: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Caveolin-2 Antibody (5E9E2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caveolin-2

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins (1). Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Caveolins 1 and 2 are similar in most respects, however, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell (2). Caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins (1).

Alternate Names

CAV2, Caveolin2

Gene Symbol

CAV2

Additional Caveolin-2 Products

Product Documents for Caveolin-2 Antibody (5E9E2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caveolin-2 Antibody (5E9E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for Caveolin-2 Antibody (5E9E2) - BSA Free

There are currently no reviews for this product. Be the first to review Caveolin-2 Antibody (5E9E2) - BSA Free and earn rewards!

Have you used Caveolin-2 Antibody (5E9E2) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies