Caveolin-3 Antibody

Novus Biologicals | Catalog # NB110-5029

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Caveolin-3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 14688207). Rat reactivity reported in scientific literature (PMID: 11567040).

Marker

Muscle Specific Caveolae Marker, T-tubule Marker

Specificity

Detects caveolin-3 from rat tissues. This does not detect caveolin-1 or -2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caveolin-3 Antibody

Western Blot: Caveolin-3 Antibody [NB110-5029]

Western Blot: Caveolin-3 Antibody [NB110-5029]

Western Blot: Caveolin-3 Antibody [NB110-5029] - Analysis of rat cardiac muscle protein extract.
Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029]

Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029]

Immunocytochemistry/Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - C2C11 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Caveolin 3 polyclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse skeletal muscle tissue.
Flow Cytometry: Caveolin-3 Antibody [NB110-5029]

Flow Cytometry: Caveolin-3 Antibody [NB110-5029]

Flow Cytometry: Caveolin-3 Antibody [NB110-5029] - Analysis of U-87 MG cells.
Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029]

Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029]

Immunocytochemistry/Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - HeLa cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a polyclonal anti-Caveolin 3 antibody (cat# NB110-5029) at a dilution of 1:20 overnight at 4 degrees Celsius, washed with PBS and incubated with a DyLight488 conjugated secondary antibody. Caveolin 3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse heart tissue.
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029]

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse lymph node tissue.

Applications for Caveolin-3 Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:100 - 200

Immunohistochemistry-Paraffin

1:100 - 200

Immunoprecipitation

5 ug

Western Blot

1 - 3 ug/ml
Application Notes
WB: Detects an approx. 21 kDa protein representing caveolin-3 from rat heart homogenate.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caveolin-3

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.

Alternate Names

CAV3, Caveolin3, LGMD1C, LQT9, M-caveolin

Entrez Gene IDs

859 (Human); 12391 (Mouse); 29161 (Rat)

Gene Symbol

CAV3

UniProt

P56539

Additional Caveolin-3 Products

Product Documents for Caveolin-3 Antibody

Certificate of Analysis

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Product Specific Notices for Caveolin-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caveolin-3 Antibody

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Protocols

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