CBFB Antibody - BSA Free

Chromatin Immunoprecipitation-exo-Seq: CBFB Antibody - BSA Free [NBP1-87300]

ChIP-Exo-Seq composite graph for Anti-CBFB (NBP1-87300) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Immunocytochemistry/ Immunofluorescence: CBFB Antibody - BSA Free [NBP1-87300] -

Expression of Cbfb throughout postnatal germline development. (A–D) Analysis of published scRNA-seq data from the postnatal germline development. Uniform manifold approximation projection (UMAP) representation of Cbfb transcripts (A) and germ cell subtypes (B) in the adult (P56) germline. Also, violin plots of Cbfb transcript abundance within germ cell subtypes (C) and spermatogonia through postnatal development (D) are included. (E) Representative images of immunofluorescence staining using an antibody recognizing CBF beta (green) and DAPI (grey) from testis cross-sections of adult wild-type mice. (F–I) Representative images from co-immunofluorescence staining for CBF beta (green) and select spermatogonia markers within the postnatal testis, including LIN28A [(F) undifferentiated spermatogonia], cKIT [(G) differentiating spermatogonia], GFR⍺1 [(H) SSC-enriched spermatogonia], and SOX3 [(I) progenitor-enriched spermatogonia]. White arrowhead identifies CBF beta + cells. Yellow arrowhead identifies CBF beta -cell. Scale bar is 50 um for images in (E–I). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38020932), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CBFB Antibody - BSA Free [NBP1-87300] -

Expression of Cbfb throughout postnatal germline development. (A–D) Analysis of published scRNA-seq data from the postnatal germline development. Uniform manifold approximation projection (UMAP) representation of Cbfb transcripts (A) and germ cell subtypes (B) in the adult (P56) germline. Also, violin plots of Cbfb transcript abundance within germ cell subtypes (C) and spermatogonia through postnatal development (D) are included. (E) Representative images of immunofluorescence staining using an antibody recognizing CBF beta (green) and DAPI (grey) from testis cross-sections of adult wild-type mice. (F–I) Representative images from co-immunofluorescence staining for CBF beta (green) and select spermatogonia markers within the postnatal testis, including LIN28A [(F) undifferentiated spermatogonia], cKIT [(G) differentiating spermatogonia], GFR⍺1 [(H) SSC-enriched spermatogonia], and SOX3 [(I) progenitor-enriched spermatogonia]. White arrowhead identifies CBF beta + cells. Yellow arrowhead identifies CBF beta -cell. Scale bar is 50 um for images in (E–I). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38020932), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CBFB Antibody - BSA Free [NBP1-87300] -

Expression of Cbfb throughout postnatal germline development. (A–D) Analysis of published scRNA-seq data from the postnatal germline development. Uniform manifold approximation projection (UMAP) representation of Cbfb transcripts (A) and germ cell subtypes (B) in the adult (P56) germline. Also, violin plots of Cbfb transcript abundance within germ cell subtypes (C) and spermatogonia through postnatal development (D) are included. (E) Representative images of immunofluorescence staining using an antibody recognizing CBF beta (green) and DAPI (grey) from testis cross-sections of adult wild-type mice. (F–I) Representative images from co-immunofluorescence staining for CBF beta (green) and select spermatogonia markers within the postnatal testis, including LIN28A [(F) undifferentiated spermatogonia], cKIT [(G) differentiating spermatogonia], GFR⍺1 [(H) SSC-enriched spermatogonia], and SOX3 [(I) progenitor-enriched spermatogonia]. White arrowhead identifies CBF beta + cells. Yellow arrowhead identifies CBF beta -cell. Scale bar is 50 um for images in (E–I). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38020932), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CBFB Antibody - BSA Free [NBP1-87300] -

Expression of Cbfb throughout postnatal germline development. (A–D) Analysis of published scRNA-seq data from the postnatal germline development. Uniform manifold approximation projection (UMAP) representation of Cbfb transcripts (A) and germ cell subtypes (B) in the adult (P56) germline. Also, violin plots of Cbfb transcript abundance within germ cell subtypes (C) and spermatogonia through postnatal development (D) are included. (E) Representative images of immunofluorescence staining using an antibody recognizing CBF beta (green) and DAPI (grey) from testis cross-sections of adult wild-type mice. (F–I) Representative images from co-immunofluorescence staining for CBF beta (green) and select spermatogonia markers within the postnatal testis, including LIN28A [(F) undifferentiated spermatogonia], cKIT [(G) differentiating spermatogonia], GFR⍺1 [(H) SSC-enriched spermatogonia], and SOX3 [(I) progenitor-enriched spermatogonia]. White arrowhead identifies CBF beta + cells. Yellow arrowhead identifies CBF beta -cell. Scale bar is 50 um for images in (E–I). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38020932), licensed under a CC-BY license. Not internally tested by Novus Biologicals.