CC2D1A Antibody - BSA Free
Novus Biologicals | Catalog # NB500-255
![Western Blot: CC2D1A Antibody [NB500-255]](https://resources.rndsystems.com/images/products/CC2D1A-Antibody-Western-Blot-NB500-255-img0001.jpg "Western Blot: CC2D1A Antibody [NB500-255]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Coiled-coil and C2 Domain Containing 1A using the numbering given in TrEMBL entry Q6P1N0 (GeneID 54862).
Specificity
NB500-255 is specific for human TOUF/FLJ-20241 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CC2D1A Antibody - BSA Free (NB500-255) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-CC2D1A Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CC2D1A Antibody - BSA Free
Western Blot: CC2D1A Antibody [NB500-255]
Western Blot: CC2D1A Antibody [NB500-255] - Detection of Human TOUF/FLJ20241 on HeLa whole cell lysate using NB500-255.![Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255]](https://resources.rndsystems.com/images/products/CC2D1A-Antibody-Immunohistochemistry-Paraffin-NB500-255-img0002.jpg "Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255]")
Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255]
Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255] - Human colon carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).![Western Blot: CC2D1A Antibody [NB500-255]](https://resources.rndsystems.com/images/products/CC2D1A-Antibody-Western-Blot-NB500-255-img0008.jpg "Western Blot: CC2D1A Antibody [NB500-255]")
Western Blot: CC2D1A Antibody [NB500-255]
Western Blot: CC2D1A Antibody [NB500-255] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cellsApplications for CC2D1A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
1 -4 ug/mg lysate
Western Blot
1:1000 - 1:10000
Application Notes
This antibody can be used in Western Blot, Immunofluorescence, and Immunoprecipitation. Recommended Starting Dilutions*: WB: 1:1,000-1:10,000 IP: 1-4 ug/mg lysate IF: 1/100 *Optimal dilutions should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CC2D1A
Alternate Names
Akt kinase-interacting protein 1, coiled-coil and C2 domain containing 1A, coiled-coil and C2 domain-containing protein 1A, Five repressor element under dual repression-binding protein 1, FLJ20241, FLJ41160, FRE under dual repression-binding protein 1, FREUD-1, mental retardation, nonsyndromic, autosomal recessive, 3, MRT3Freud-1/Aki1, Putative NF-kappa-B-activating protein 023N, putative NFkB activating protein
Entrez Gene IDs
54862 (Human)
Gene Symbol
CC2D1A
UniProt
Additional CC2D1A Products
Product Documents for CC2D1A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CC2D1A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CC2D1A Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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