Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CCDC5 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CCDC5 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC5 Antibody - Azide and BSA Free

CCDC5 Antibody - Azide and BSA Free CCDC5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] -

Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] - Immunofluorescence staining of CCDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-49164 (dilution ratio 1:200) at 4C overnight. Then cells were stained with Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CCDC5 Antibody - Azide and BSA Free CCDC5 Antibody - Azide and BSA Free

Western Blot: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] -

Western Blot: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] - Lane A: HL60 Whole Cell Lysate. Lane B: U937 Whole Cell Lysate. Lane C: MOLT4 Whole Cell Lysate. Lane D: Daudi Whole Cell Lysate Lysates/proteins at 30 ug per lane using NBP3-49164 at 1:500 dilution. Secondary: HRP-conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa

Applications for CCDC5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS (pH 7.0)

Format

Azide and BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC5

CCDC5 belongs to the HAUS1 family. More specifically, CCDC5 is a component of the HAUS augmen complex. Originally founded in Drosophila, the complex plays an important role in spindle assembly, maintenance of centrosome integrity and completion of cytokinesis.

Alternate Names

CCDC5Enhancer of invasion-cluster, coiled-coil domain containing 5 (spindle associated), FLJ40084, HAUS augmin-like complex subunit 1, HAUS augmin-like complex, subunit 1, HEI-CCoiled-coil domain-containing protein 5, HEICFLJ21094, HsT1461

Gene Symbol

HAUS1

Additional CCDC5 Products

Product Documents for CCDC5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CCDC5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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