CCL2/MCP1 Antibody (ECE.2)

Novus Biologicals | Catalog # NBP1-42312

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoassay

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # ECE.2
View all CCL2/MCP1 Primary Antibodies »

Product Specifications

Immunogen

Murine CCL2/MCP1

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for CCL2/MCP1 Antibody (ECE.2)

Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312]

Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312]

Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.

Applications for CCL2/MCP1 Antibody (ECE.2)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The antibody is useful for detection of MCP-1 by Western blot. Furthermore it can be used in immunoassays. The typical starting working dilution is 1:10. It is recommended that users test the reagent and determine their own optimal dilutions. Although not tested this antibody may be useful in immunohistochemistry on frozen and paraffin sections. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26413869)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCL2/MCP1

Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine-beta family of cytokines. The protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. It may play a inprotant role in the initiation and/or progression of pulmonary hypertension (PH). Blockade of a systemic MCP-1 signal pathway in vivo may prevent PH.

Alternate Names

C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF

Gene Symbol

CCL2

Additional CCL2/MCP1 Products

Product Documents for CCL2/MCP1 Antibody (ECE.2)

Certificate of Analysis

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Product Specific Notices for CCL2/MCP1 Antibody (ECE.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCL2/MCP1 Antibody (ECE.2)

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Protocols

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