CCL3/CCL4 Antibody (93321) [PE]
Novus Biologicals | Catalog # FAB270P
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Neutralization, Immunocytochemistry
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 93321
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Product Specifications
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP-1 alpha in direct ELISAs and
Western blots. In direct ELISAs, greater than 75% cross-reactivity with
recombinant human (rh) CCL4/MIP-1 beta is observed while approximately 30%
cross-reactivity with recombinant mouse (rm) CCL3, rmCCL4/MIP-1 beta, and rhCCL14
is observed. No cross-reactivity with rhCCL1, 2, 5, 7, 8, 11, 13, 15, 16, 17,
18, 19, 20, 21, 22, 23, 24, 25, 26, rmCCL1, 2, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12,
17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for CCL3/CCL4 Antibody (93321) [PE]
Application
Recommended Usage
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCL3/CCL4
Long Name
CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive
Alternate Names
C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3
Gene Symbol
CCL3
Additional CCL3/CCL4 Products
Product Documents for CCL3/CCL4 Antibody (93321) [PE]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CCL3/CCL4 Antibody (93321) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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