CCR8 Antibody - BSA Free

Novus Biologicals | Catalog # NLS3847

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CCR8 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from C-terminus of human CCR8.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (95%), Gibbon (89%), Monkey (89%), Marmoset (84%).

Specificity

Human CCR8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for CCR8 Antibody - BSA Free

Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody [NLS3847] - Brain Pituitary Pars Anterior
Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody [NLS3847] - Analysis of anti-CCR8 antibody with tonsil, germinal center at 22 ug/ ml.
Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody - BSA Free [NLS3847]

Immunohistochemistry-Paraffin: CCR8 Antibody [NLS3847] - Analysis of anti-CCR8 antibody with human lymph node, non-Hodgkins lymphoma.

Applications for CCR8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

22 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR8

CCR8, a Chemokine Receptor, binds the CC-chemokine I-309, which is secreted by umbilical vein endothelial cells. CCR8 also binds viral monocyte inflammatory protein-I (vMIP-I), which is induced by Kaposi sarcoma-related human herpesvirus-8. CCR8 induces endothelial chemotaxis and may function as an HIV-1 co-receptor on primary human cells, contributing to HIV-1-induced thymic pathogenesis (Lee et al., 2000). CCR8 expression has been reported in spleen and thymus, natural-killer cells, monocytes, and T cells, and at lower levels in peripheral blood leukocytes. Increased expression has been seen in allergen-challenged atopic asthma. No CCR8 expression was found in mesangial cells of the kidney cortex. ESTs have been isolated from human normal kidney, eye, and B cell/lung/testis libraries and from human brain, germ cell, and pancreas cancer libraries.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human)

Gene Symbol

CCR8

UniProt

P51685

Additional CCR8 Products

Product Documents for CCR8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCR8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCR8 Antibody - BSA Free

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Protocols

View specific protocols for CCR8 Antibody - BSA Free (NLS3847):

Immunohistochemistry Protocol for CCR8 Antibody (NLS3847):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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