CD117/c-kit Antibody (DMC446) - Chimeric

Name: CD117/c-kit Antibody (DMC446) - Chimeric
Brand: Novus Biologicals
SKU: NBP3-48343
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48343

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # DMC446

View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

CD117/c-kit recombinant protein expressed in mammalian cells (Accession# P10721). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CD117/c-kit Antibody (DMC446) - Chimeric

Flow Cytometry: CD117/c-kit Antibody (DMC446) - Chimeric [NBP3-48343]

Flow cytometry analysis of antigen binding of anti-human CD117 mAb(NBP3-48343). (A) NBP3-48343 does not bind to A431 cells that do not express CD117. (B) A clear peak shift of NBP3-48343 was seen compared to the control when incubated with CD117-expressing TT cells, indicating strong binding of NBP3-48343 to CD117. Antibodies were incubated at 5 ug/mL.

Flow Cytometry: CD117/c-kit Antibody (DMC446) - Chimeric [NBP3-48343]

Flow cytometry analysis with Anti-CD117/c-kit/c-kit (DMC446) on HEK293 cells transfected with human CD117/c-kit/c-kit (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Applications for CD117/c-kit Antibody (DMC446) - Chimeric

Application

Recommended Usage

Flow Cytometry

1:100

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (DMC446) - Chimeric

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD117/c-kit Antibody (DMC446) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.