CD11a/CD18 Antibody (24) - Azide Free

Novus Biologicals | Catalog # NBP3-11272

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Frozen, Immunoassay, Flow Cytometry, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24

Format

Azide Free
View all CD11a/CD18 Primary Antibodies »

Product Specifications

Immunogen

Fibronectin-purified human monocytes

Specificity

Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<24 EU/mg

Applications for CD11a/CD18 Antibody (24) - Azide Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts activated CD11/CD18 to be inhibited.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD11a/CD18

The monoclonal antibody 24 recognizes an epitope of 174 kD present on leukocyte integrins, CD11/CD18 (LFA-1) or beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding. The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody 24 prevents "deadhesion" of receptor/ligand pairs, possible locking leukocyte integrins in an "active" conformation.

Alternate Names

CD11 antigen-like family member A, CD11a antigen, CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide, integrin alpha-L, integrin gene promoter, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide), Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, LFA-1, LFA-1 alpha, LFA1A, LFA-1A, lymphocyte function-associated antigen 1

Gene Symbol

ITGAL

Additional CD11a/CD18 Products

Product Documents for CD11a/CD18 Antibody (24) - Azide Free

Certificate of Analysis

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Product Specific Notices for CD11a/CD18 Antibody (24) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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