Q: Is the epitope for antibody clone BU15 intracellular or extracellular?

A: The epitope for this antibody is extracellular.

CD11c Antibody (BU15) - BSA Free

Novus Biologicals | Catalog # NBP1-45018

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Citations (1)

Product Documents

Clone BU15 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # BU15

Format

BSA Free

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Product Specifications

Immunogen

Dendritic cells of synovial fluid

Marker

Dendritic Cell Marker

Specificity

The antibody BU15 reacts with CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages. HLDA III; WS Code M 256 HLDA V; WS Code AS S143 HLDA VI; WS Code AS Ref.6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD11c Antibody (BU15) - BSA Free (NBP1-45018) is a monoclonal antibody validated for use in IHC, Flow and IP. Anti-CD11c Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD11c Antibody (BU15) - BSA Free

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) Pacific BlueTM.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP (monocyte gate).

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) APC.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP-CyTM5.5.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE-CyTM7..

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) FITC.

Flow Cytometry: CD11c Antibody (BU15) - BSA Free [NBP1-45018]

Separation of human monocytes (red-filled) from CD11c negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human CD11c (BU15) purified antibody (concentration in sample 2 ug/ml, GAM APC).

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Applications for CD11c Antibody (BU15) - BSA Free

Application

Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50

Application Notes

This antibody is Cytof ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD11c

CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

Additional CD11c Products

Product Documents for CD11c Antibody (BU15) - BSA Free

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Product Specific Notices for CD11c Antibody (BU15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.