CD11c Antibody (CA11.6A1) - BSA Free

Novus Biologicals | Catalog # NB100-65291

Novus Biologicals
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Key Product Details

Species Reactivity

Canine

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CA11.6A1

Format

BSA Free
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Product Specifications

Immunogen

Made to Dog CD11c

Reactivity Notes

Predicted cross-reactivities: Hooded Seal, Raccoon

Marker

Dendritic Cell Marker

Specificity

Recognizes the canine CD11c cell surface antigen, a member of the alpha integrin family. Canine CD11c is expressed by monocytes, granulocytes and by dendritic cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD11c Antibody (CA11.6A1) - BSA Free (NB100-65291) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD11c Antibody (CA11.6A1) - BSA Free

Flow Cytometry: CD11c Antibody (CA11.6A1) [NB100-65291]

Flow Cytometry: CD11c Antibody (CA11.6A1) [NB100-65291]

Flow Cytometry: CD11c Antibody (CA11.6A1) [NB100-65291] - Figure A. PE conjugated Mouse anti Canine CD45 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD45 and purified Mouse anti Canine CD11c detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on monocytes.

Applications for CD11c Antibody (CA11.6A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Reviewed Applications

Read 1 review rated 2 using NB100-65291 in the following applications:

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Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11c

CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukemia.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

Additional CD11c Products

Product Documents for CD11c Antibody (CA11.6A1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD11c Antibody (CA11.6A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD11c Antibody (CA11.6A1) - BSA Free (1)

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Customer Images


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  • CD11c Antibody (CA11.6A1)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 12/08/2017
    NB100-65291 was tested at 1:10-400 dilution as recommended, with incubation for overnight at 4C degree, and Alexa488 conjugated antibody was used. Image was captured with epifluorescent microscope.
    CD11c Antibody (CA11.6A1) - BSA Free NB100-65291

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