CD133-A15, 5'-DY647

Catalog # Availability Size / Price Qty
6103/50U
CD133-A15, 5'-DY647 | Fluorescent Cell Surface Markers
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Description: High affinity fluorescently tagged aptamer for CD133 (AC133 epitope)
Product Details
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Biological Activity

High affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.

For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.

Application of CD133-A15, 5'-DY647 in Immunocytochemistry

Application of CD133-A15, 5'-DY647 in Immunocytochemistry

Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.

Image kindly provided by Birte Aggeler, Bio-Techne.

Technical Data

Aptamer type (DNA/RNA)
RNA
Number of Bases
15
Sequence Modifications
2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag
M.Wt:
5549.58
Formula:
C182H219F9N58O104P16
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the patent holder, Deakin University.

Background References

  1. RNA aptamers targeting cancer stem cell marker CD133.
    Shigdar et al.
    Cancer Lett., 2013;330:84

Product Datasheets

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Reconstitution Calculator

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