Biological ActivityHigh affinity fluorescently tagged aptamer for CD133 (AC133 epitope) (Kd values are 33.9 and 83.2 nM in Hep3B and HT29 cells respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.
For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Application of CD133-A15, 5'-DY647 in Immunocytochemistry
Aptamer applied at 100 nM concentration to HT29 cells. Magnification 40x.
Image kindly provided by Birte Aggeler, Bio-Techne.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
RNA aptamers targeting cancer stem cell marker CD133.
Shigdar et al.
Cancer Lett., 2013;330:84
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